Cellapoptosisandtumoroccurrenceanddevelopmentarecloselyrelated,itforthemaintenanceoftissuesandorgansofnormalmorphologyandfunctionplaysanimportantroleinthetumor,treatmentandpreventionintheprocessofgreatsignificance.
Becausetheessenceherbalhassmalltoxicandsideeffects,andtherapeuticeffectisstableandaseriesofadvantages,therefore,iscommittedtotheChineseherbalmedicinescreeningofanticancerdrugshasbecometheresearchdirectionofnewcentury.Asaresultofresveratrolhasantibacterial,anti-inflammatory,theresistanceofcancercellstogrowwaitforacharacteristic,thereforeinthetreatmentofcancerisofgreatscientificresearchprospect.
Objective:thisexperimentinprimaryhumanlungadenocarcinomaA549cellsastheresearchobject,inordertounderstandresveratrolinduceslungcancerA549cellapoptosis.Methods:byinvertedmicroscopy,fluorescencemicroscopytoobservetheeffectsofdifferentconcentrationsofresveratroleffectsonA549cellsin24h,48hand72hcellgrowthpattern,atthesametime,usingthetrypanbluedyeexclusionassayforthedetectionofresveratrolonA549cellgrowthinhibitionrate,andthendeterminethevalueofIC50,andcellapoptosis,andthenthroughtheflowcytometryincellcycleandcellmitochondrialmembranepotentialchange.ResultsshowthatresveratroleffectsinhumanlungcancercellA54948hshowdifferentdegreesofapoptosistraits,whiledisplayingthedose-effectrelationship.ResveratrolinducedapoptosisofA549in48hoptimalconcentrationswere110µg/mL,80µg/mL,andtheIC50valueswere108.002µg/mL,77.462µg/mL.Afterthefinalflowcytometryresults,resveratrolmaybeA549celllagstopsatSphaseandG2phase,andthemitochondrialmembranepotentialdecreaseswithconcentration.Conclusion:ResveratrolinducesapoptosisinhumanlungadenocarcinomaA549cellapoptosis,thevaluesofIC50were108.002μg/mL,77.462μg/mL.Mitochondrialmembranepotentialdecreaseswithincreasingconcentration.AndcellcyclearrestinG2phaseandSphase,andinducingcellapoptosis.