医学英语分子生物学实验,英译汉ComparethecDNAsequenceof4variants,thereareonlydifferencesbetweenexon3andexon7,thusdesignspecificprimerforthesetwoexonscoulddifferentiate4variants.1.totalRNAextraction2.RT-PCR:primer

　　医学英语分子生物学实验,英译汉

　　ComparethecDNAsequenceof4variants,thereareonlydifferencesbetweenexon3andexon7,thusdesignspecificprimerforthesetwoexonscoulddifferentiate4variants.

　　1.totalRNAextraction

　　2.RT-PCR:

　　primerforexon3:Tm:about52°C

　　senseprimer:5'GATTGGACAGGCTGGACT3'

　　anti-senseprimer:5'AGAAGTGGTGGCAGTGATT3'

　　predictedresults:288bpproductinNurr1-a,b,c;noamplifiedproductinNurr1-d.

　　primerforexon7:Tm:58-59°C

　　senseprimer:5'CCAAAGCCGACCAAGACC3'

　　anti-senseprimer:5'ATTATTGCTGGCGGTGGC3'

　　predictedresults:472bpproductinNurr1-a,d;418bpproductinNurr1-b;351bpproductinNurr1-c.

　　3.resultanalysis:

　　Nurr1-aNurr1-bNurr1-cNurr1-d

　　amplicationforexon3288bp288bp288bpnoproduct

　　amplicationforexon7472bp418bp351bp472bp

　　4.

　　Problem:RefSeqforNurr1-b,c,dhavebeenpermanentlysuppressedbecauseofinsufficientsupport.