医学英语分子生物学实验,英译汉ComparethecDNAs-查字典问答网
分类选择

来自钱世栋的问题

  医学英语分子生物学实验,英译汉ComparethecDNAsequenceof4variants,thereareonlydifferencesbetweenexon3andexon7,thusdesignspecificprimerforthesetwoexonscoulddifferentiate4variants.1.totalRNAextraction2.RT-PCR:primer

  医学英语分子生物学实验,英译汉

  ComparethecDNAsequenceof4variants,thereareonlydifferencesbetweenexon3andexon7,thusdesignspecificprimerforthesetwoexonscoulddifferentiate4variants.

  1.totalRNAextraction

  2.RT-PCR:

  primerforexon3:Tm:about52°C

  senseprimer:5'GATTGGACAGGCTGGACT3'

  anti-senseprimer:5'AGAAGTGGTGGCAGTGATT3'

  predictedresults:288bpproductinNurr1-a,b,c;noamplifiedproductinNurr1-d.

  primerforexon7:Tm:58-59°C

  senseprimer:5'CCAAAGCCGACCAAGACC3'

  anti-senseprimer:5'ATTATTGCTGGCGGTGGC3'

  predictedresults:472bpproductinNurr1-a,d;418bpproductinNurr1-b;351bpproductinNurr1-c.

  3.resultanalysis:

  Nurr1-aNurr1-bNurr1-cNurr1-d

  amplicationforexon3288bp288bp288bpnoproduct

  amplicationforexon7472bp418bp351bp472bp

  4.

  Problem:RefSeqforNurr1-b,c,dhavebeenpermanentlysuppressedbecauseofinsufficientsupport.

1回答
2019-05-04 09:13
我要回答
请先登录
蔡玉华

  比较了四个变体的cDNA序列,它们只在外显子3和外显子7之间存在区别,因此可以通过设计针对这两个外显子的特殊引物来区别这四个变体.1.总RNA提取2.逆转录PCR外显子3引物:熔解温度:约52℃正向引物5'GATTGGACAGGCTGGA...

2019-05-04 09:17:09

最新问答

推荐文章

猜你喜欢

附近的人在看

推荐阅读

拓展阅读

  • 大家都在看
  • 小编推荐
  • 猜你喜欢
  •