英语翻译2.6.ExpressiblewaterTheamo-查字典问答网

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　　英语翻译2.6.ExpressiblewaterTheamountofexpressiblewater(EW)foreachtreatmentwasmeasured.Samplesof3g(±0.2g)ofﬁshgelswereweighedandputbetweentwolayersofﬁlterpaper.Sampleswereplacedatthebottomof50ml

　　英语翻译

　　2.6.Expressiblewater

　　Theamountofexpressiblewater(EW)foreachtreatmentwasmeasured.Samplesof3g(±0.2g)ofﬁshgelswereweighedandputbetweentwolayersofﬁlterpaper.Sampleswereplacedatthebottomof50mlcentrifugetubesandcentrifugedat1000gfor15minat15°C(Uresti,Ramı´rez,Lo´pez-Arias,&Va´zquez,2003).Immediatelyaftercentrifugation,theﬁshgelsampleswereweighedandtheEWwascalculatedas:expressiblewater(%)=(Wi-Wf)/Wi*100,whereWiistheinitialweightofgel,Wfistheﬁnalweightofgel.

　　2.7.TBAtest

　　MethodwasbasedonGomes,Silva,Nascimento,andFukuma(2003).TheTBA(2-thiobarbituricacid)solutionwaspreparedbyweighing0.3gofTBAandtransferringina100mlbeakerwith90mldistilledwater.Thebeakerwasplacedinawaterbath(80°C)untilcompletedissolution.Thesolutionwasthenquantitativelytransferredtoa100mlvolumetricﬂaskandthevolumecompletedwithdistilledwatersoastoachievea0.021MTBAsolution.Mincedsample(50g)wasblendedaftertheadditionof6mlofethanolicsolutionofbutylatedhydroxytoluene(BHT,1g/l)topreventautoxidation.Aliquotsofhomogenizedsample(10g)weretransferredtoaﬂat-bottomed

　　ﬂaskandonedropofsiliconeanti-foamingagentaddedplus2.5mlof4NHCland97.5mlofdistilledwater.Thissamplewasthendistilledandtheﬁrst50mlofdistillatecollected.Distillationwascarriedoutintriplicate.Then5mlofthedistillateplus0.6mlofBHT(1g/l)wereaddedto5mlof0.021MTBAsolutionintoascrew-captesttubeandheatedinawaterbath(90°C)for40minforpinkcolordevelopment.Thetesttubewasthencooledandtheopticaldensitywasdeterminedat532nmonaspectrophotometer(Spectrum-cn722E,China)usingcontrolsolutioncontaining5mldistilledwater,5mlTBAsolutionand0.6mlBHT.TBAvalueswereexpressedasmgofmalondialdehyde(MDA)perkgofsample.Theconcentrationof

　　MDAwascalculatedfromastandardcurveusing1,1,3,3-tetraethoxy-propane(TEP)asthestandardcompound.

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2019-06-24 01:33

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孙军帅

　　2.6.可表现的水

　　给每治疗的大量可表现水(EW)是标准的.3g(±0.2g)的样品sh凝胶是秤重和放在二之间层lter纸.样品被放置在那底部50毫升离心分离机管和离心分离机在1000g15分钟在15°C(Uresti,公羊?′rez,瞧!′pez-抒情调,&伏-安培′zquez,2003).在离心分离之后立刻,那sh凝胶样品是秤重和EW是有计划的当做:可表现的水(%)=(Wi-Wf)/Wi*100,哪里Wi是那起始凝胶的重量,Wf是那nal凝胶的重量.

　　2.7.TBA测试

　　方法以Gomes、Silva、Nascimento和Fukuma为基础.(2003)TBA(2thiobarbituric的酸)解决是准备被TBA的秤重0.3g而且以被蒸馏的90毫升在一个100毫升大口杯中转移水.大口杯是放在一之内水沭浴(80°C)直到完全的分解.解决然后数量地被转移到一100毫升测定体积的为了要达成这0.021MTBA解决,问和用蒸馏的水体积.切碎样品(50g)被混合了在避免自然氧化的butylatedhydroxytoluene(BHT,1g/l)的6毫升ethanolic解决的附加之后.均质化的样品(10g)的能整除是转移到一在-根据

　　问和一下降矽树脂反起泡沫代理人附加的加号蒸馏的2.5毫升的4NHCl和97.5毫升水.这个样品然后蒸馏和那rst50毫升的distillate收集成的.蒸馏是被运的在一式三份.然后5毫升distillate加号0.6毫升的BHT(1g/l)是附加的至0.021MTBA解决的5毫升进入一之内螺丝钉-无边帽测试管和热的在一水沭浴(90°C)为40分钟为粉红的彩色发展.测试管然后是冷却和光学的密度是坚决的在使用的一个分光光度计(光谱-cn的722E,中国)上的532个nm控制包含5毫升的解决蒸馏水,5毫升TBA解决和0.6毫升BHT.TBA价值是表达当做malondialdehyde(MDA)的毫克每一公斤样品.集中

　　MDA是有计划的从标准曲线使用1,1,3,3tetraethoxy的-丙烷(TEP)当做标准化合物.

2019-06-24 01:37:28

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